Determining Cell Proliferation & Viability

Corporate researchers evaluated ASEA Redox Supplement on proliferation cell counts of human cells and associated markers for cell viability and health.

Study Protocol

Human lung microvascular endothelial cells (HMVEC-L) received treatment of a 5 – 20% concentration of ASEA Redox Supplement for 72 hours. A control group received a 20% phosphate buffered saline solution treatment. Researchers used a Coulter counter—an apparatus for counting and sizing particles suspended in electrolytes—to determine cell count. Serum LDH levels measured cell culture viability at 0 – 20% ASEA Redox Supplement serum concentrations. Similar experiments were performed for murine (JB6) epidermal cells.

Results Summary

High concentrations (5 – 20% volume/volume percent) of ASEA Redox Supplement exposure inhibited cellular proliferation for both HMVEC-L and JB6 cell types (determined from cell counts). The HMVEC-L inhibition was clearly concentration dependent, with a 20% loss of cell count at 20% ASEA Redox Supplement concentration. In contrast to decreased proliferation, serum LDH levels significantly decreased with ASEA Redox Supplement concentration between 5 – 20%, indicating increased cell membrane integrity. The results seem to suggest that cellular proliferation decreases while membrane viability increases at high levels.